Description
Chemical Identity
IGF-1 LR3 (Long R3 Insulin-like Growth Factor-1) is an 83-amino acid analog of human IGF-1 with the molecular formula C400H625N111O115S9 and a molecular weight of 9111.4 Da (CAS number 946870-92-4). The compound features two key modifications from native IGF-1: an arginine substitution at position 3 (replacing glutamic acid) and a 13-amino acid N-terminal extension peptide derived from methionyl porcine growth hormone.
These modifications significantly reduce binding to the six known IGF binding proteins (IGFBPs), which normally sequester over 99% of circulating IGF-1 and regulate its bioavailability. The result is greatly enhanced free IGF-1 receptor activation and biological potency compared to native IGF-1. IGF-1 LR3 is also referred to as Long R3 IGF-1 or Long Arg3 IGF-1.
| Property | Value |
|---|---|
| Molecular Formula | C400H625N111O115S9 |
| Molecular Weight | 9111.4 Da |
| CAS Number | 946870-92-4 |
| Structure | 83 amino acid IGF-1 analog with Arg3 substitution and 13-aa N-terminal extension |
| Research Status | Preclinical |
Research Overview
IGF-1 LR3 has been investigated in preclinical research spanning cell biology, tissue engineering, and growth factor pharmacology. The primary mechanism involves activation of the IGF-1 receptor (IGF-1R), a receptor tyrosine kinase that signals through the PI3K/Akt and MAPK/ERK pathways to promote cellular proliferation, differentiation, and survival while inhibiting apoptosis.
The dramatically reduced IGFBP binding results in approximately 2-3 fold greater potency than native IGF-1 in cell culture systems, making IGF-1 LR3 the preferred form for in vitro research applications where consistent, maximal IGF-1 receptor activation is desired.
Key areas of published research include:
- Cell Culture and Tissue Engineering: IGF-1 LR3 is widely used as a cell culture supplement in biopharmaceutical manufacturing and tissue engineering, where its enhanced bioavailability and resistance to IGFBP sequestration provide more consistent and potent growth factor stimulation than native IGF-1.
- IGF-1 Receptor Signaling: The compound serves as a valuable research tool for studying IGF-1R activation, downstream PI3K/Akt signaling, and the biological consequences of sustained IGF-1 receptor stimulation in the absence of IGFBP-mediated regulation.
- IGFBP Biology: IGF-1 LR3’s reduced IGFBP binding makes it useful for dissecting the relative contributions of IGF-1 receptor activation versus IGFBP-dependent effects in various biological systems.
IGF-1 LR3 is a preclinical research tool with no regulatory approvals for therapeutic use.
Specifications
All IGF-1 LR3 supplied by Prescott Bio Canada is manufactured to research-grade standards.
| Specification | Detail |
|---|---|
| Purity | >98% (HPLC) |
| Form | Lyophilized powder |
| Appearance | White to off-white powder |
| Storage (lyophilized) | -20°C, protected from light |
| Storage (reconstituted) | 2-8°C, use within 30 days |
| Solubility | Soluble in sterile water or appropriate buffer (slightly acidic pH recommended) |
Each vial is accompanied by a Certificate of Analysis (COA) documenting purity, identity, and endotoxin testing.
Research Applications
IGF-1 LR3 is supplied exclusively for in vitro and in vivo laboratory research. Published experimental applications include:
- Cell culture supplementation and biopharmaceutical manufacturing
- IGF-1 receptor signaling and PI3K/Akt pathway research
- Tissue engineering and regenerative medicine studies
- IGFBP biology and IGF-1 bioavailability research
- Cellular proliferation, differentiation, and survival studies
- Comparative studies with native IGF-1 and other growth factors
Researchers should consult the primary literature and institutional review protocols before designing experiments with IGF-1 LR3.
Storage and Handling
Lyophilized powder: Store at -20°C in the original sealed vial, protected from light and moisture. Under these conditions, IGF-1 LR3 maintains stability for up to 24 months.
Reconstituted solution: Reconstitute with sterile water or slightly acidic buffer (pH 3-4) for optimal stability. For cell culture use, dilute into culture medium immediately before use. Store reconstituted IGF-1 LR3 at 2-8°C and use within 30 days. Avoid repeated freeze-thaw cycles.
Handling precautions: This product is intended for research use only (RUO). Not for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices including appropriate PPE.