Description
Chemical Identity
5-Amino-1MQ is a small molecule inhibitor with the molecular formula C10H11N2+ (as the cation) and a molecular weight of approximately 159.21 Da. Based on a quinolinium scaffold, 5-Amino-1MQ features a methylated nitrogen at position 1 and an amino group at position 5. Unlike most compounds on this site, 5-Amino-1MQ is not a peptide but a small organic molecule that selectively inhibits nicotinamide N-methyltransferase (NNMT), an enzyme that degrades nicotinamide and reduces NAD+ availability through the methyl sink pathway.
5-Amino-1MQ is also known by the following designations: 5-amino-1-methylquinolinium and NNMTi.
| Property | Value |
|---|---|
| Molecular Formula | C10H11N2+ |
| Molecular Weight | 159.21 Da |
| CAS Number | 42464-96-0 |
| Type | Small molecule NNMT inhibitor (quinolinium) |
| Research Status | Preclinical |
Research Overview
5-Amino-1MQ has been investigated in preclinical models of obesity, metabolic disease, aging, and muscle function. The mechanism of action involves selective inhibition of NNMT, an enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing 1-methylnicotinamide. By blocking this methylation, 5-Amino-1MQ conserves nicotinamide for recycling through the NAD+ salvage pathway, effectively increasing intracellular NAD+ levels through a mechanism distinct from NAD+ precursors like NMN or NR.
Key areas of published research include:
- Anti-Obesity Effects: In diet-induced obese mice, 5-Amino-1MQ administration produced significant reductions in body weight, white adipose tissue mass, and adipocyte size without affecting food intake. The compound suppressed lipogenesis in adipocytes through NNMT inhibition, establishing a link between the methyl sink pathway and adipose tissue metabolism. These findings suggest that NNMT is a druggable target for obesity research.
- Muscle Function and Aging: In aged mice, 5-Amino-1MQ combined with exercise improved grip strength by up to 60% compared to exercise alone. This preclinical finding suggests that NNMT inhibition may enhance the beneficial effects of physical activity on muscle function during aging, potentially through restored NAD+ availability in skeletal muscle.
- NAD+ Pathway Modulation: By conserving nicotinamide rather than supplying exogenous precursors, 5-Amino-1MQ offers a complementary approach to NAD+ replenishment strategies. NNMT is overexpressed in white adipose tissue and in multiple cancer types, making it a target of interest across metabolic and oncologic research domains.
All published evidence for 5-Amino-1MQ comes from animal and cell culture studies. No human clinical trials have been published or registered.
Specifications
All 5-Amino-1MQ supplied by Prescott Bio Canada is manufactured to research-grade standards.
| Specification | Detail |
|---|---|
| Purity | >98% (HPLC) |
| Form | Lyophilized powder |
| Appearance | White to off-white powder |
| Storage (lyophilized) | -20°C, protected from light |
| Storage (reconstituted) | 2-8°C, use within 30 days |
| Solubility | Soluble in bacteriostatic water, sterile water, or DMSO |
Each vial is accompanied by a Certificate of Analysis (COA) documenting purity, identity, and endotoxin testing.
Research Applications
5-Amino-1MQ is supplied exclusively for in vitro and in vivo laboratory research. Published experimental applications include:
- Investigation of NNMT enzyme biology and the nicotinamide methyl sink pathway in metabolic regulation
- Study of NAD+ salvage pathway modulation through enzyme inhibition rather than precursor supplementation
- Examination of adipocyte biology, lipogenesis suppression, and white adipose tissue remodeling
- Research into age-related muscle function decline and exercise potentiation through metabolic pathway restoration
Researchers should consult the primary literature and institutional review protocols before designing experiments with 5-Amino-1MQ.
Storage and Handling
Lyophilized powder: Store at -20°C in the original sealed vial, protected from light and moisture. Under these conditions, 5-Amino-1MQ maintains stability for up to 24 months.
Reconstituted solution: Reconstitute with sterile water, DMSO, or an appropriate solvent to the desired concentration. Store reconstituted 5-Amino-1MQ at 2-8°C and use within 30 days. Avoid repeated freeze-thaw cycles.
Handling precautions: This product is intended for research use only (RUO). Not for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices including appropriate PPE.