Description
Chemical Identity
SNAP-8 analytical reference compound. Molecular weight 1075.16 Da. Also known as: Acetyl Octapeptide-3, Acetyl Glutamyl Heptapeptide-1, SNAP8. Research status: Preclinical.
Research Overview
SNAP-8 (Acetyl Octapeptide-3) is a synthetic octapeptide that acts as a competitive antagonist of the SNARE complex, mimicking the N-terminal end of SNAP-25. By inhibiting neurotransmitter release at the neuromuscular junction, it reduces muscle contraction and is used in topical cosmeceutical formulations to diminish expression wrinkles.
SNAP-8 acts as a competitive antagonist of the SNARE complex, the molecular machinery that mediates synaptic vesicle fusion and neurotransmitter release at the neuromuscular junction. Its mechanism involves several key steps:
Normal neuromuscular signaling requires the assembly of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) complex, consisting of three proteins:
Clinical evaluations of SNAP-8 have demonstrated measurable anti-wrinkle effects when applied topically:
Key published studies on SNAP-8 include: “Method development for acetyl octapeptide-3 analysis by liquid chromatography-tandem mass spectrometry” (Journal of Analytical Science and Technology, 2020); “Cosmeceutical Peptides in the Framework of Sustainable Wellness Economy” (Frontiers in Chemistry, 2020); “The anti-wrinkle efficacy of argireline in Chinese subjects: a randomized placebo-controlled study” (Journal of Cosmetic and Laser Therapy, 2013). These findings should be interpreted within the context of the experimental models and conditions described in each publication.
Research Context
SNAP-8, known chemically as Acetyl Octapeptide-3, is a synthetic peptide developed by the Spanish biotechnology company Lipotec (now part of Lubrizol Life Science) as a topical cosmeceutical ingredient for reducing expression wrinkles. It is an extension of the earlier hexapeptide Argireline (Acetyl Hexapeptide-3/8), with two additional amino acids (alanine and aspartate) added to the sequence, yielding an octapeptide with enhanced anti-wrinkle activity.
SNAP-8 belongs to the class of neurotransmitter inhibitor peptides — synthetic sequences designed to mimic portions of proteins involved in neuromuscular junction signaling. By interfering with the molecular machinery required for neurotransmitter release, these peptides attenuate the muscle contractions responsible for dynamic facial wrinkles such as crow’s feet and forehead lines.
1. SNAP-25 (Synaptosomal-Associated Protein, 25 kDa): Located on the presynaptic membrane 2. Syntaxin-1: Also on the presynaptic membrane 3. VAMP/Synaptobrevin: Located on the synaptic vesicle membrane
Specifications
| Sequence | Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 |
| Molecular Weight | 1075.16 Da |
| Molecular Formula | C41H70N16O16S |
| CAS Number | 868844-74-0 |
| Purity | >=98% (HPLC) |
| Appearance | White to off-white lyophilized powder |
| Format | Lyophilized powder, sterile filtered |
| Solubility | Soluble in bacteriostatic water, sterile water, or normal saline |
| Storage | Store at -20°C (lyophilized). Reconstituted: 2-8°C, use within 30 days |
| Shipping | Ambient temperature (stable in lyophilized form) |
Each lot is accompanied by a Certificate of Analysis (COA) documenting purity, identity, and endotoxin testing results.
Research Applications
SNAP-8 reference compound has been documented in the published scientific literature across the following in vitro and preclinical research areas:
- The SNARE Complex
- How SNAP-8 Intervenes
- Wrinkle Reduction Studies
- Skin Penetration Challenges
Researchers are advised to consult the primary literature for detailed experimental protocols, concentrations, and conditions relevant to their specific area of investigation involving SNAP-8.
Storage and Handling
SNAP-8 is supplied as a lyophilized powder and should be stored at -20°C upon receipt for long-term stability. Protect from light, moisture, and repeated temperature fluctuations. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture absorption.
For reconstitution, add sterile water or an appropriate buffer slowly along the vial wall to avoid foaming. Gently swirl to dissolve — do not vortex. Reconstituted SNAP-8 solutions should be stored at 2-8°C and used within 30 days. Aliquoting is recommended to minimize freeze-thaw cycles. Consult the Safety Data Sheet (SDS) for detailed handling and disposal guidance.
For laboratory research use only (in vitro). Not for human or animal use. Not for diagnostic, therapeutic, or clinical purposes. SNAP-8 is supplied as an analytical reference compound for use by qualified research personnel at accredited institutions. Prescott Bio Canada does not provide guidance on administration, dosing, or use in living organisms.